Stimulated Raman Scattering (SRS) is a label-free technique utilized to analyze the chemical nature of various substances with high-speed. However, SRS can be affected by parasitic effects that can distort the signal. In order to eliminate these spurious effects, we propose a frequency-modulation SRS scheme realized for the first time with an acousto-optic tunable filter, which can perform measurements from the fingerprint to the CH-stretch region of the spectrum without any modification of the optical setup. We demonstrate the efficient cancellation of the parasitic effects with our developed configuration on several samples: human hairs, polymer beads and human cells.

Presenter: Elissa Grassi, King Abdullah Univ. of Science and Technology (Saudi Arabia)

Author(s): Elissa Grassi, King Abdullah Univ. of Science and Technology (Saudi Arabia); Sergey Laptenok, King Abdullah Univ. of Science and Technology (Saudi Arabia); Luca Genchi, King Abdullah Univ. of Science and Technology (Saudi Arabia); Carlo Liberale, King Abdullah Univ. of Science and Technology (Saudi Arabia)

Source Name: SPIE, Paper 11973 - 42

Source URL: https://spie.org/photonics-west/presentation/Frequency-modulation-stimulated-Raman-scattering-microscopy-with-an-acousto-optic/11973-42?enableBackToBrowse=true